| 01 May 2001 | 1st Round | Bedfordshire v Nottinghamshire Cricket Board | Wardown Park, Luton | Results |
| 01 May 2001 | 1st Round | Kent Cricket Board v Hampshire Cricket Board | Mote Park, Maidstone | Results |
| 01 May 2001 | 1st Round | Lancashire Cricket Board v Yorkshire Cricket Board | Seedhill, Nelson | Results |
| 01 May 2001 | 1st Round | Lincolnshire v Suffolk | London Road, Sleaford | Results |
| 01 May 2001 | 1st Round | Middlesex Cricket Board v Northumberland | John Walker's Ground, Southgate | Results |
| 01 May 2001 | 1st Round | Oxfordshire v Huntingdonshire | Vicarage Hill, East Challow | Results |
| 01 May 2001 | 1st Round | Shropshire v Devon | London Road, Shrewsbury | Results |
| 01 May 2001 | 1st Round | Somerset Cricket Board v Wales Minor Counties | Willis Lane, North Perrott | Results |
| 01 May 2001 | 1st Round | Staffordshire v Worcestershire Cricket Board | Knutton Road, Wolstanton | Results |
| 01 May 2001 | 1st Round | Wiltshire v Derbyshire Cricket Board | Hardenhuish Park, Chippenham | Results |
| 15 May 2001 | 2nd Round | Cambridgeshire v Derbyshire Cricket Board | The Avenue Sports Club Ground, March | Results |
| 15 May 2001 | 2nd Round | Cornwall v Cheshire | Roskear, Camborne | Results |
| 15 May 2001 | 2nd Round | Dorset v Bedfordshire | Dean Park, Bournemouth | Results |
| 15 May 2001 | 2nd Round | Herefordshire v Gloucestershire Cricket Board | The Parks, Brockhampton | Results |
| 15 May 2001 | 2nd Round | Hertfordshire v Durham Cricket Board | Digswell Park, Welwyn Garden City | Results |
| 15 May 2001 | 2nd Round | Kent Cricket Board v Buckinghamshire | Mote Park, Maidstone | Results |
| 15 May 2001 | 2nd Round | Middlesex Cricket Board v Berkshire | Old Deer Park, Richmond | Results |
| 15 May 2001 | 2nd Round | Norfolk v Wales Minor Counties | Manor Park, Horsford | Results |
| 15 May 2001 | 2nd Round | Northamptonshire Cricket Board v Yorkshire Cricket Board | County Ground, Northampton | Results |
| 15 May 2001 | 2nd Round | Suffolk v Essex Cricket Board | Wamil Way, Mildenhall | Results |
| 15 May 2001 | 2nd Round | Surrey Cricket Board v Huntingdonshire | Peaches Close, Cheam | Results |
| 15 May 2001 | 2nd Round | Sussex Cricket Board v Shropshire | Horntye Park, Hastings | Results |
| 15 May 2001 | 2nd Round | Warwickshire Cricket Board v Leicestershire Cricket Board | Bulls Head Ground, Coventry | Results |
| 15 May 2001 | 2nd Round | Worcestershire Cricket Board v Cumberland | Chester Road North, Kidderminster | Results |
| 27 Jun 2001 | 3rd Round | Bedfordshire v Yorkshire | Wardown Park, Luton | Results |
| 27 Jun 2001 | 3rd Round | Berkshire v Essex | Sonning Lane, Reading | Results |
| 27 Jun 2001 | 3rd Round | Cambridgeshire v Somerset | The Avenue Sports Club Ground, March | Results |
| 27 Jun 2001 | 3rd Round | Cornwall v Sussex | Boscawen Park, Truro | Results |
| 27 Jun 2001 | 3rd Round | Cumberland v Kent | Ernest Pass Memorial Ground, Barrow-in-Furness | Results |
| 27 Jun 2001 | 3rd Round | Durham v Hampshire | Seat Unique Riverside Ground, Chester-le-Street | Results |
| 27 Jun 2001 | 3rd Round | Glamorgan v Derbyshire | Sophia Gardens, Cardiff | Results |
| 27 Jun 2001 | 3rd Round | Herefordshire v Middlesex | Mortimer Park, Kingsland | Results |
| 27 Jun 2001 | 3rd Round | Hertfordshire v Worcestershire | Balls Park, Hertford | Results |
| 27 Jun 2001 | 3rd Round | Kent Cricket Board v Warwickshire | Spitfire Ground, St Lawrence, Canterbury | Results |
| 27 Jun 2001 | 3rd Round | Lancashire v Warwickshire Cricket Board | Stanley Park, Blackpool | Results |
| 27 Jun 2001 | 3rd Round | Northamptonshire Cricket Board v Northamptonshire | County Ground, Northampton | Results |
| 27 Jun 2001 | 3rd Round | Suffolk v Nottinghamshire | Wamil Way, Mildenhall | Results |
| 27 Jun 2001 | 3rd Round | Surrey Cricket Board v Surrey | Woodbridge Road, Guildford | Results |
| 27 Jun 2001 | 3rd Round | Sussex Cricket Board v Gloucestershire | Cricket Field Road, Horsham | Results |
| 27 Jun 2001 | 3rd Round | Wales Minor Counties v Leicestershire | St Helen's, Swansea | Results |
| 11 Jul 2001 | 4th Round | Gloucestershire v Durham | Seat Unique Stadium, Bristol | Results |
| 11 Jul 2001 | 4th Round | Kent v Northamptonshire | Spitfire Ground, St Lawrence, Canterbury | Results |
| 11 Jul 2001 | 4th Round | Lancashire v Sussex | Emirates Old Trafford, Manchester | Results |
| 11 Jul 2001 | 4th Round | Nottinghamshire v Leicestershire | Trent Bridge, Nottingham | Results |
| 11 Jul 2001 | 4th Round | Somerset v Glamorgan | The Cooper Associates County Ground, Taunton | Results |
| 11 Jul 2001 | 4th Round | Warwickshire v Essex | Edgbaston, Birmingham | Results |
| 11 Jul 2001 | 4th Round | Worcestershire v Herefordshire | Visit Worcestershire New Road, Worcester | Results |
| 11 Jul 2001 | 4th Round | Yorkshire v Surrey | Headingley, Leeds | Results |
| 24 Jul 2001 | Quarter-Final | Worcestershire v Leicestershire | Visit Worcestershire New Road, Worcester | Results |
| 25 Jul 2001 | Quarter-Final | Kent v Somerset | Spitfire Ground, St Lawrence, Canterbury | Results |
| 25 Jul 2001 | Quarter-Final | Lancashire v Durham | Stanley Park, Blackpool | Results |
| 25 Jul 2001 | Quarter-Final | Yorkshire v Warwickshire | Headingley, Leeds | Results |
| 11 Aug 2001 | Semi-Final | Somerset v Warwickshire | The Cooper Associates County Ground, Taunton | Results |
| 12 Aug 2001 | Semi-Final | Leicestershire v Lancashire | Uptonsteel County Ground, Leicester | Results |
| 01 Sep 2001 | Final | Leicestershire v Somerset | Lord's Cricket Ground, St John's Wood | Results |
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