| 08 May 2000 | D Zone | Derbyshire Second XI v Northamptonshire Second XI | North Road, Glossop | Results |
| 11 May 2000 | A Zone | Minor Counties Under-25s v Glamorgan Second XI | The County Ground, Swindon | Results |
| 15 May 2000 | C Zone | Hampshire Second XI v Somerset Second XI | County Ground, Southampton | Results |
| 19 May 2000 | A Zone | Minor Counties Under-25s v Leicestershire Second XI | Lancot Park, Totternhoe | Results |
| 22 May 2000 | B Zone | Worcestershire Second XI v Yorkshire Second XI | Visit Worcestershire New Road, Worcester | Results |
| 25 May 2000 | A Zone | Minor Counties Under-25s v Nottinghamshire Second XI | London Road, Sleaford | Results |
| 26 May 2000 | A Zone | Nottinghamshire Second XI v Minor Counties Under-25s | Station Lane, Farnsfield | Results |
| 30 May 2000 | B Zone | Warwickshire Second XI v Yorkshire Second XI | Arlington Avenue, Leamington Spa | Results |
| 01 Jun 2000 | B Zone | Warwickshire Second XI v Worcestershire Second XI | Sandwell Park, West Bromwich | Results |
| 02 Jun 2000 | A Zone | Glamorgan Second XI v Minor Counties Under-25s | Sophia Gardens, Cardiff | Results |
| 05 Jun 2000 | A Zone | Leicestershire Second XI v Gloucestershire Second XI | Leicester Road, Hinckley | Results |
| 05 Jun 2000 | D Zone | Northamptonshire Second XI v Derbyshire Second XI | Stowe School Ground, Stowe | Results |
| 12 Jun 2000 | D Zone | Essex Second XI v Kent Second XI | The Cloud County Ground, Chelmsford | Results |
| 12 Jun 2000 | C Zone | Hampshire Second XI v Marylebone Cricket Club Young Cricketers | County Ground, Southampton | Results |
| 12 Jun 2000 | D Zone | Middlesex Second XI v Northamptonshire Second XI | Corfton Road, Ealing | Results |
| 12 Jun 2000 | A Zone | Nottinghamshire Second XI v Gloucestershire Second XI | Worksop College Ground, Worksop | Results |
| 19 Jun 2000 | D Zone | Middlesex Second XI v Essex Second XI | Payne's Folly, Harrow | Results |
| 19 Jun 2000 | C Zone | Somerset Second XI v Marylebone Cricket Club Young Cricketers | The Cooper Associates County Ground, Taunton | Results |
| 26 Jun 2000 | D Zone | Essex Second XI v Northamptonshire Second XI | The Toby Howe Cricket Ground, Billericay | Results |
| 26 Jun 2000 | A Zone | Gloucestershire Second XI v Glamorgan Second XI | Seat Unique Stadium, Bristol | Results |
| 26 Jun 2000 | A Zone | Leicestershire Second XI v Nottinghamshire Second XI | Leicester Road, Hinckley | Results |
| 26 Jun 2000 | C Zone | Surrey Second XI v Somerset Second XI | The Kia Oval, Kennington | Results |
| 26 Jun 2000 | C Zone | Sussex Second XI v Hampshire Second XI | Sea Lane, Middleton-on-Sea | Results |
| 27 Jun 2000 | D Zone | Kent Second XI v Northamptonshire Second XI | Spitfire Ground, St Lawrence, Canterbury | Results |
| 27 Jun 2000 | A Zone | Nottinghamshire Second XI v Leicestershire Second XI | Oakfeld Lane, Warsop | Results |
| 27 Jun 2000 | B Zone | Yorkshire Second XI v Worcestershire Second XI | Clifton Park, York | Results |
| 28 Jun 2000 | B Zone | Durham Second XI v Warwickshire Second XI | Ashbrooke, Sunderland | Results |
| 28 Jun 2000 | B Zone | Lancashire Second XI v Yorkshire Second XI | Seedhill, Nelson | Results |
| 28 Jun 2000 | A Zone | Leicestershire Second XI v Glamorgan Second XI | Leicester Road, Hinckley | Results |
| 28 Jun 2000 | C Zone | Surrey Second XI v Hampshire Second XI | The Kia Oval, Kennington | Results |
| 29 Jun 2000 | D Zone | Derbyshire Second XI v Middlesex Second XI | Racecourse Ground, Derby | Results |
| 29 Jun 2000 | B Zone | Lancashire Second XI v Durham Second XI | Green Lane, Bolton | Results |
| 29 Jun 2000 | A Zone | Minor Counties Under-25s v Gloucestershire Second XI | White Post Road, Bodicote | Results |
| 29 Jun 2000 | D Zone | Northamptonshire Second XI v Kent Second XI | County Ground, Northampton | Results |
| 29 Jun 2000 | A Zone | Nottinghamshire Second XI v Glamorgan Second XI | Dale Field, Collingham | Results |
| 30 Jun 2000 | D Zone | Derbyshire Second XI v Kent Second XI | Racecourse Ground, Derby | Results |
| 30 Jun 2000 | A Zone | Leicestershire Second XI v Minor Counties Under-25s | Leicester Road, Hinckley | Results |
| 30 Jun 2000 | D Zone | Northamptonshire Second XI v Middlesex Second XI | Orlingbury Road, Isham | Results |
| 30 Jun 2000 | C Zone | Somerset Second XI v Hampshire Second XI | The Cooper Associates County Ground, Taunton | Results |
| 30 Jun 2000 | B Zone | Worcestershire Second XI v Warwickshire Second XI | Visit Worcestershire New Road, Worcester | Results |
| 30 Jun 2000 | B Zone | Yorkshire Second XI v Durham Second XI | Wagon Lane, Bingley | Results |
| 03 Jul 2000 | A Zone | Glamorgan Second XI v Gloucestershire Second XI | Sophia Gardens, Cardiff | Results |
| 03 Jul 2000 | C Zone | Hampshire Second XI v Sussex Second XI | Utilita Bowl (Nursery), Southampton | Results |
| 03 Jul 2000 | D Zone | Middlesex Second XI v Kent Second XI | Old Deer Park, Richmond | Results |
| 03 Jul 2000 | D Zone | Northamptonshire Second XI v Essex Second XI | Campbell Park, Milton Keynes | Results |
| 03 Jul 2000 | C Zone | Somerset Second XI v Surrey Second XI | The Cooper Associates County Ground, Taunton | Results |
| 03 Jul 2000 | B Zone | Warwickshire Second XI v Durham Second XI | Edgbaston, Birmingham | Results |
| 03 Jul 2000 | B Zone | Worcestershire Second XI v Lancashire Second XI | Visit Worcestershire New Road, Worcester | Results |
| 04 Jul 2000 | A Zone | Glamorgan Second XI v Nottinghamshire Second XI | Spytty Park, Newport | Results |
| 04 Jul 2000 | B Zone | Lancashire Second XI v Warwickshire Second XI | Moseley Road, Fallowfield | Results |
| 04 Jul 2000 | C Zone | Somerset Second XI v Sussex Second XI | The Cooper Associates County Ground, Taunton | Results |
| 04 Jul 2000 | B Zone | Worcestershire Second XI v Durham Second XI | Main Road, Ombersley | Results |
| 05 Jul 2000 | A Zone | Gloucestershire Second XI v Nottinghamshire Second XI | Bristol University Sports Ground, Bristol | Results |
| 05 Jul 2000 | C Zone | Hampshire Second XI v Surrey Second XI | County Ground, Southampton | Results |
| 05 Jul 2000 | D Zone | Kent Second XI v Derbyshire Second XI | Three Hills Sports Park, Folkestone | Results |
| 06 Jul 2000 | B Zone | Durham Second XI v Yorkshire Second XI | Park Drive, Hartlepool | Results |
| 06 Jul 2000 | A Zone | Gloucestershire Second XI v Leicestershire Second XI | Bristol University Sports Ground, Bristol | Results |
| 06 Jul 2000 | D Zone | Kent Second XI v Essex Second XI | Three Hills Sports Park, Folkestone | Results |
| 06 Jul 2000 | C Zone | Marylebone Cricket Club Young Cricketers v Somerset Second XI | Uxbridge Cricket Club Ground, Uxbridge | Results |
| 06 Jul 2000 | D Zone | Middlesex Second XI v Derbyshire Second XI | Arden Field, Finchley | Results |
| 06 Jul 2000 | C Zone | Sussex Second XI v Surrey Second XI | New County Ground, Hove | Results |
| 06 Jul 2000 | B Zone | Warwickshire Second XI v Lancashire Second XI | Bulls Head Ground, Coventry | Results |
| 07 Jul 2000 | B Zone | Durham Second XI v Worcestershire Second XI | Wood Terrace, South Shields | Results |
| 07 Jul 2000 | D Zone | Essex Second XI v Derbyshire Second XI | Anglo-American Playing Fields, Saffron Walden | Results |
| 07 Jul 2000 | A Zone | Glamorgan Second XI v Leicestershire Second XI | Spytty Park, Newport | Results |
| 07 Jul 2000 | C Zone | Marylebone Cricket Club Young Cricketers v Surrey Second XI | Uxbridge Cricket Club Ground, Uxbridge | Results |
| 07 Jul 2000 | C Zone | Sussex Second XI v Somerset Second XI | New County Ground, Hove | Results |
| 10 Jul 2000 | B Zone | Durham Second XI v Lancashire Second XI | Hornby Park, Seaton Carew | Results |
| 10 Jul 2000 | D Zone | Essex Second XI v Middlesex Second XI | Ashwells Road, Brentwood | Results |
| 10 Jul 2000 | C Zone | Marylebone Cricket Club Young Cricketers v Hampshire Second XI | Uxbridge Cricket Club Ground, Uxbridge | Results |
| 10 Jul 2000 | C Zone | Surrey Second XI v Sussex Second XI | Cheam Road, Sutton | Results |
| 10 Jul 2000 | B Zone | Yorkshire Second XI v Warwickshire Second XI | St George's Road, Harrogate | Results |
| 11 Jul 2000 | C Zone | Marylebone Cricket Club Young Cricketers v Sussex Second XI | Uxbridge Cricket Club Ground, Uxbridge | Results |
| 11 Jul 2000 | B Zone | Yorkshire Second XI v Lancashire Second XI | Savile Park, Castleford | Results |
| 13 Jul 2000 | D Zone | Kent Second XI v Middlesex Second XI | Spitfire Ground, St Lawrence, Canterbury | Results |
| 13 Jul 2000 | B Zone | Lancashire Second XI v Worcestershire Second XI | The Racecourse, Radcliffe | Results |
| 14 Jul 2000 | C Zone | Sussex Second XI v Marylebone Cricket Club Young Cricketers | Horntye Park, Hastings | Results |
| 17 Jul 2000 | C Zone | Surrey Second XI v Marylebone Cricket Club Young Cricketers | The Kia Oval, Kennington | Results |
| 24 Jul 2000 | D Zone | Derbyshire Second XI v Essex Second XI | Repton School Ground, Repton | Results |
| 28 Jul 2000 | A Zone | Gloucestershire Second XI v Minor Counties Under-25s | Seat Unique Stadium, Bristol | Results |
| 07 Aug 2000 | Semi-Final | Hampshire Second XI v Durham Second XI | Utilita Bowl (Nursery), Southampton | Results |
| 07 Aug 2000 | Semi-Final | Kent Second XI v Leicestershire Second XI | Spitfire Ground, St Lawrence, Canterbury | Results |
| 04 Sep 2000 | Final | Hampshire Second XI v Leicestershire Second XI | County Ground, Southampton | Results |
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