| 04 May 1959 | | Glamorgan Second XI v Gloucestershire Second XI | The Gnoll, Neath | Results |
| 04 May 1959 | | Warwickshire Second XI v Leicestershire Second XI | Weddington Road, Nuneaton | Results |
| 06 May 1959 | | Yorkshire Second XI v Warwickshire Second XI | Memorial Park, Mirfield | Results |
| 07 May 1959 | | Glamorgan Second XI v Worcestershire Second XI | Rodney Parade, Newport | Results |
| 07 May 1959 | | Gloucestershire Second XI v Surrey Second XI | Seat Unique Stadium, Bristol | Results |
| 08 May 1959 | | Northamptonshire Second XI v Somerset Second XI | County Ground, Northampton | Results |
| 11 May 1959 | | Hampshire Second XI v Gloucestershire Second XI | County Ground, Southampton | Results |
| 11 May 1959 | | Northamptonshire Second XI v Derbyshire Second XI | County Ground, Northampton | Results |
| 11 May 1959 | | Surrey Second XI v Warwickshire Second XI | The Kia Oval, Kennington | Results |
| 13 May 1959 | | Leicestershire Second XI v Northamptonshire Second XI | Lime Kilns Ground, Oakham | Results |
| 13 May 1959 | | Somerset Second XI v Worcestershire Second XI | The Cooper Associates County Ground, Taunton | Results |
| 13 May 1959 | | Sussex Second XI v Middlesex Second XI | New County Ground, Hove | Results |
| 13 May 1959 | | Warwickshire Second XI v Nottinghamshire Second XI | Arlington Avenue, Leamington Spa | Results |
| 16 May 1959 | | Surrey Second XI v Kent Second XI | The Kia Oval, Kennington | Results |
| 18 May 1959 | | Derbyshire Second XI v Leicestershire Second XI | Christchurch Meadows, Belper | Results |
| 18 May 1959 | | Somerset Second XI v Hampshire Second XI | Combe Park, Bath | Results |
| 18 May 1959 | | Warwickshire Second XI v Northamptonshire Second XI | Edgbaston, Birmingham | Results |
| 18 May 1959 | | Yorkshire Second XI v Lancashire Second XI | Queens, Scarborough | Results |
| 20 May 1959 | | Nottinghamshire Second XI v Leicestershire Second XI | Main Road, Wollaton | Results |
| 21 May 1959 | | Glamorgan Second XI v Warwickshire Second XI | St Helen's, Swansea | Results |
| 21 May 1959 | | Sussex Second XI v Worcestershire Second XI | Central Recreation Ground, Hastings | Results |
| 25 May 1959 | | Gloucestershire Second XI v Hampshire Second XI | Seat Unique Stadium, Bristol | Results |
| 25 May 1959 | | Northamptonshire Second XI v Kent Second XI | Desborough Road, Rothwell | Results |
| 25 May 1959 | | Nottinghamshire Second XI v Warwickshire Second XI | Trent Bridge, Nottingham | Results |
| 25 May 1959 | | Worcestershire Second XI v Somerset Second XI | Visit Worcestershire New Road, Worcester | Results |
| 27 May 1959 | | Derbyshire Second XI v Yorkshire Second XI | Queen's Park, Chesterfield | Results |
| 27 May 1959 | | Essex Second XI v Middlesex Second XI | Overton Drive, Wanstead | Results |
| 27 May 1959 | | Lancashire Second XI v Nottinghamshire Second XI | Emirates Old Trafford, Manchester | Results |
| 27 May 1959 | | Leicestershire Second XI v Somerset Second XI | Aylestone Road, Leicester | Results |
| 01 Jun 1959 | | Warwickshire Second XI v Yorkshire Second XI | Edgbaston, Birmingham | Results |
| 01 Jun 1959 | | Worcestershire Second XI v Northamptonshire Second XI | Visit Worcestershire New Road, Worcester | Results |
| 03 Jun 1959 | | Derbyshire Second XI v Nottinghamshire Second XI | Rutland Recreation Ground, Ilkeston | Results |
| 03 Jun 1959 | | Lancashire Second XI v Glamorgan Second XI | Emirates Old Trafford, Manchester | Results |
| 03 Jun 1959 | | Northamptonshire Second XI v Yorkshire Second XI | County Ground, Northampton | Results |
| 03 Jun 1959 | | Sussex Second XI v Kent Second XI | New County Ground, Hove | Results |
| 04 Jun 1959 | | Gloucestershire Second XI v Worcestershire Second XI | Rissington Road, Bourton-on-the-Water | Results |
| 05 Jun 1959 | | Northamptonshire Second XI v Leicestershire Second XI | County Ground, Northampton | Results |
| 05 Jun 1959 | | Sussex Second XI v Surrey Second XI | New County Ground, Hove | Results |
| 08 Jun 1959 | | Hampshire Second XI v Sussex Second XI | United Services Ground, Portsmouth | Results |
| 10 Jun 1959 | | Kent Second XI v Middlesex Second XI | Mote Park, Maidstone | Results |
| 10 Jun 1959 | | Leicestershire Second XI v Gloucestershire Second XI | Aylestone Road, Leicester | Results |
| 10 Jun 1959 | | Northamptonshire Second XI v Worcestershire Second XI | County Ground, Northampton | Results |
| 10 Jun 1959 | | Surrey Second XI v Essex Second XI | The Kia Oval, Kennington | Results |
| 13 Jun 1959 | | Middlesex Second XI v Sussex Second XI | Lord's Cricket Ground, St John's Wood | Results |
| 15 Jun 1959 | | Gloucestershire Second XI v Glamorgan Second XI | Erinoid Ground, Stroud | Results |
| 15 Jun 1959 | | Hampshire Second XI v Somerset Second XI | County Ground, Southampton | Results |
| 15 Jun 1959 | | Lancashire Second XI v Northamptonshire Second XI | Emirates Old Trafford, Manchester | Results |
| 15 Jun 1959 | | Worcestershire Second XI v Kent Second XI | Visit Worcestershire New Road, Worcester | Results |
| 17 Jun 1959 | | Essex Second XI v Kent Second XI | John H Burrows Recreation Ground, Hadleigh | Results |
| 17 Jun 1959 | | Worcestershire Second XI v Lancashire Second XI | Visit Worcestershire New Road, Worcester | Results |
| 17 Jun 1959 | | Yorkshire Second XI v Northamptonshire Second XI | Fartown, Huddersfield | Results |
| 22 Jun 1959 | | Kent Second XI v Gloucestershire Second XI | London Transport Sports Ground, Beckenham | Results |
| 22 Jun 1959 | | Northamptonshire Second XI v Lancashire Second XI | Stewart and Lloyds Sports Club Ground, Corby | Results |
| 22 Jun 1959 | | Somerset Second XI v Glamorgan Second XI | Agricultural Showgrounds, Frome | Results |
| 22 Jun 1959 | | Surrey Second XI v Middlesex Second XI | The Kia Oval, Kennington | Results |
| 22 Jun 1959 | | Warwickshire Second XI v Worcestershire Second XI | Edgbaston Foundation Community Sports Ground, Birmingham | Results |
| 24 Jun 1959 | | Middlesex Second XI v Kent Second XI | Teddington Cricket Club Ground, Bushy Park, Teddington | Results |
| 24 Jun 1959 | | Warwickshire Second XI v Lancashire Second XI | Edgbaston, Birmingham | Results |
| 29 Jun 1959 | | Sussex Second XI v Hampshire Second XI | New County Ground, Hove | Results |
| 01 Jul 1959 | | Derbyshire Second XI v Warwickshire Second XI | Racecourse Ground, Derby | Results |
| 01 Jul 1959 | | Glamorgan Second XI v Somerset Second XI | The Island Ground, Barry Island | Results |
| 01 Jul 1959 | | Kent Second XI v Hampshire Second XI | Cobdown Sports Ground, Aylesford | Results |
| 01 Jul 1959 | | Middlesex Second XI v Leicestershire Second XI | Lincoln Road, Enfield | Results |
| 02 Jul 1959 | | Lancashire Second XI v Worcestershire Second XI | Emirates Old Trafford, Manchester | Results |
| 06 Jul 1959 | | Kent Second XI v Surrey Second XI | Hesketh Park, Dartford | Results |
| 06 Jul 1959 | | Somerset Second XI v Northamptonshire Second XI | The Cooper Associates County Ground, Taunton | Results |
| 08 Jul 1959 | | Kent Second XI v Northamptonshire Second XI | The Grove, Sittingbourne | Results |
| 08 Jul 1959 | | Leicestershire Second XI v Middlesex Second XI | Aylestone Road, Leicester | Results |
| 08 Jul 1959 | | Nottinghamshire Second XI v Derbyshire Second XI | Elm Avenue, Newark-on-Trent | Results |
| 13 Jul 1959 | | Derbyshire Second XI v Lancashire Second XI | North Road, Glossop | Results |
| 13 Jul 1959 | | Nottinghamshire Second XI v Yorkshire Second XI | Town Ground, Worksop | Results |
| 13 Jul 1959 | | Surrey Second XI v Gloucestershire Second XI | The Cricket Green, Mitcham | Results |
| 13 Jul 1959 | | Worcestershire Second XI v Leicestershire Second XI | Visit Worcestershire New Road, Worcester | Results |
| 15 Jul 1959 | | Kent Second XI v Essex Second XI | Bat and Ball Ground, Gravesend | Results |
| 15 Jul 1959 | | Northamptonshire Second XI v Middlesex Second XI | County Ground, Northampton | Results |
| 15 Jul 1959 | | Worcestershire Second XI v Warwickshire Second XI | Seth Somers Park, Halesowen | Results |
| 17 Jul 1959 | | Northamptonshire Second XI v Surrey Second XI | County Ground, Northampton | Results |
| 20 Jul 1959 | | Somerset Second XI v Kent Second XI | Clarence Park, Weston-super-Mare | Results |
| 20 Jul 1959 | | Warwickshire Second XI v Surrey Second XI | Griff and Coton Ground, Nuneaton | Results |
| 20 Jul 1959 | | Worcestershire Second XI v Sussex Second XI | Visit Worcestershire New Road, Worcester | Results |
| 21 Jul 1959 | | Gloucestershire Second XI v Leicestershire Second XI | Victoria Ground, Cheltenham | Results |
| 22 Jul 1959 | | Derbyshire Second XI v Northamptonshire Second XI | Racecourse Ground, Derby | Results |
| 22 Jul 1959 | | Hampshire Second XI v Middlesex Second XI | Dean Park, Bournemouth | Results |
| 23 Jul 1959 | | Somerset Second XI v Leicestershire Second XI | Backwell Flax Bourton Cricket Club Ground, Flax Bourton | Results |
| 27 Jul 1959 | | Essex Second XI v Northamptonshire Second XI | Bayard Recreation Ground, Brightlingsea | Results |
| 27 Jul 1959 | | Yorkshire Second XI v Nottinghamshire Second XI | Woodlands Ground, Brodsworth | Results |
| 29 Jul 1959 | | Glamorgan Second XI v Lancashire Second XI | Cardiff Arms Park, Cardiff | Results |
| 29 Jul 1959 | | Middlesex Second XI v Hampshire Second XI | The Paulin Ground, Winchmore Hill | Results |
| 30 Jul 1959 | | Northamptonshire Second XI v Essex Second XI | County Ground, Northampton | Results |
| 01 Aug 1959 | | Worcestershire Second XI v Glamorgan Second XI | Visit Worcestershire New Road, Worcester | Results |
| 03 Aug 1959 | | Lancashire Second XI v Yorkshire Second XI | Emirates Old Trafford, Manchester | Results |
| 03 Aug 1959 | | Leicestershire Second XI v Derbyshire Second XI | Uptonsteel County Ground, Leicester | Results |
| 03 Aug 1959 | | Northamptonshire Second XI v Warwickshire Second XI | County Ground, Northampton | Results |
| 05 Aug 1959 | | Lancashire Second XI v Derbyshire Second XI | Emirates Old Trafford, Manchester | Results |
| 07 Aug 1959 | | Surrey Second XI v Sussex Second XI | The Kia Oval, Kennington | Results |
| 10 Aug 1959 | | Essex Second XI v Sussex Second XI | New Road, Mistley | Results |
| 10 Aug 1959 | | Kent Second XI v Worcestershire Second XI | Private Banks Sports Ground, Catford | Results |
| 12 Aug 1959 | | Warwickshire Second XI v Glamorgan Second XI | Edgbaston Foundation Community Sports Ground, Birmingham | Results |
| 12 Aug 1959 | | Kent Second XI v Sussex Second XI | Wellesley House School Ground, Broadstairs | Results |
| 12 Aug 1959 | | Middlesex Second XI v Essex Second XI | John Walker's Ground, Southgate | Results |
| 14 Aug 1959 | | Sussex Second XI v Essex Second XI | Central Recreation Ground, Hastings | Results |
| 17 Aug 1959 | | Gloucestershire Second XI v Kent Second XI | Seat Unique Stadium, Bristol | Results |
| 17 Aug 1959 | | Lancashire Second XI v Warwickshire Second XI | Emirates Old Trafford, Manchester | Results |
| 17 Aug 1959 | | Leicestershire Second XI v Nottinghamshire Second XI | Coventry Road, Lutterworth | Results |
| 17 Aug 1959 | | Surrey Second XI v Northamptonshire Second XI | Beddington Park, Wallington | Results |
| 17 Aug 1959 | | Yorkshire Second XI v Derbyshire Second XI | Coatham Road, Redcar | Results |
| 19 Aug 1959 | | Hampshire Second XI v Kent Second XI | May's Bounty, Basingstoke | Results |
| 19 Aug 1959 | | Leicestershire Second XI v Warwickshire Second XI | Wyggeston Grammar School Ground, Leicester | Results |
| 19 Aug 1959 | | Middlesex Second XI v Northamptonshire Second XI | The Paulin Ground, Winchmore Hill | Results |
| 19 Aug 1959 | | Nottinghamshire Second XI v Lancashire Second XI | Steetley Company Ground, Shireoaks | Results |
| 22 Aug 1959 | | Middlesex Second XI v Surrey Second XI | Lord's Cricket Ground, St John's Wood | Results |
| 24 Aug 1959 | | Kent Second XI v Somerset Second XI | Cheriton Road Sports Ground, Folkestone | Results |
| 24 Aug 1959 | | Warwickshire Second XI v Derbyshire Second XI | General Electric Company Ground, Coventry | Results |
| 26 Aug 1959 | | Essex Second XI v Surrey Second XI | Forest Side, Chingford | Results |
| 01 Sep 1959 | | Leicestershire Second XI v Worcestershire Second XI | Uptonsteel County Ground, Leicester | Results |
| 03 Sep 1959 | | Worcestershire Second XI v Gloucestershire Second XI | Visit Worcestershire New Road, Worcester | Results |
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