| 26 May 2012 | First Round | Outcasts v Louth Second XI | Hirst Priory Park, Crowle | Results |
| 26 May 2012 | First Round | Nettleham v Cleethorpes Second XI | Mulsanne Park, Nettleham | Results |
| 26 May 2012 | First Round | Old Lincolnians v Alkborough | Lincoln Christ Hospital School Ground, Lincoln | Results |
| 26 May 2012 | First Round | Market Rasen v Hibaldstow | Rase Park, Market Rasen | Results |
| 26 May 2012 | First Round | Washingborough v Holton-le-Clay | Washingborough Playing Fields, Washingborough | Results |
| 26 May 2012 | First Round | Keelby v Haxey Second XI | Keelby Sports Ground, Keelby | Results |
| 27 May 2012 | First Round | Clee Town Laportes v Appleby Frodingham Second XI | Clee Road, Grimsby | Results |
| 26 May 2012 | First Round | Scunthorpe Town v Grimsby Town Second XI | Heslam Park, Scunthorpe | Results |
| 26 May 2012 | First Round | Immingham Blossom Way v Scothern | Blossom Way, Immingham | Results |
| 26 May 2012 | First Round | Broughton v Barton Town | Recreation Ground, Broughton | Results |
| 26 May 2012 | First Round | Horncastle v Caistor | Coronation Walk, Horncastle | Results |
| 26 May 2012 | First Round | Owmby v Hykeham | Todmoor, Owmby by Spital | Results |
| 26 May 2012 | First Round | Alford v Morton | Train Fen Holt, Alford | Results |
| 26 May 2012 | First Round | South Kelsey v Messingham | Lysaghts Sports Ground, Holton le Moor | Results |
| 26 May 2012 | First Round | Hartsholme Second XI v Cherry Willingham | Ashby Avenue, Hartsholme | Results |
| 23 Jun 2012 | Second Round | Owmby v Appleby Frodingham Second XI | Todmoor, Owmby by Spital | Results |
| 23 Jun 2012 | Second Round | Alford v Barton Town | Train Fen Holt, Alford | Results |
| 23 Jun 2012 | Second Round | Messingham v Nettleham | Memorial Playing Field, Messingham | Results |
| 23 Jun 2012 | Second Round | Holton-le-Clay v Scunthorpe Town | Station Road, Hibaldstow | Results |
| 23 Jun 2012 | Second Round | Cherry Willingham v Scothern | Playing Fields, Cherry Willingham | Results |
| 23 Jun 2012 | Second Round | Keelby v Louth Second XI | Keelby Sports Ground, Keelby | Results |
| 23 Jun 2012 | Second Round | Bracebridge Heath Second XI v Caistor | Cross O'Cliff Court, Bracebridge Heath | Results |
| 23 Jun 2012 | Second Round | Market Rasen v Alkborough | Rase Park, Market Rasen | Results |
| 22 Jul 2012 | Quarter-Final | Scunthorpe Town v Keelby | Heslam Park, Scunthorpe | Results |
| 15 Jul 2012 | Quarter-Final | Barton Town v Appleby Frodingham Second XI | Marsh Lane, Barton on Humber | Results |
| 15 Jul 2012 | Quarter-Final | Cherry Willingham v Bracebridge Heath Second XI | Playing Fields, Cherry Willingham | Results |
| 15 Jul 2012 | Quarter-Final | Alkborough v Nettleham | West Halton Lane, Alkborough | Results |
| 29 Jul 2012 | Semi-Final | Cherry Willingham v Scunthorpe Town | Playing Fields, Cherry Willingham | Results |
| 29 Jul 2012 | Semi-Final | Nettleham v Barton Town | Mulsanne Park, Nettleham | Results |
| 19 Aug 2012 | Final | Barton Town v Cherry Willingham | Marsh Lane, Barton on Humber | Results |
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