| 20 May 2007 | Preliminary Round | Ambergate v West Hallam White Rose | The Hurt Arms Ground, Ambergate | Results |
| 20 May 2007 | Preliminary Round | Belper Amateurs v Hilton | The Packhorse Ground, Alderwasley | Results |
| 20 May 2007 | Preliminary Round | Derby Congregational v Darley Dale | Brayfield Road, Littleover | Results |
| 03 Jun 2007 | First Round | Allestree v Rolls Royce | The Recreation Ground, Allestree | Results |
| 03 Jun 2007 | First Round | Barton-under-Needwood v Sawley and Long Eaton Park | Holland Park Sports Club Ground, Barton-under-Needwood | Results |
| 03 Jun 2007 | First Round | Brailsford and Ednaston v Matlock | Pools Head Lane, Brailsford | Results |
| 03 Jun 2007 | First Round | Denby Second XI v Staveley Welfare | The Copper Yard, Denby Village | Results |
| 03 Jun 2007 | First Round | Duffield v Derby Congregational | Eyes Meadow, Duffield | Results |
| 03 Jun 2007 | First Round | Heanor Town v Belper Meadows | Town Ground, Heanor | Results |
| 03 Jun 2007 | First Round | Hilton v Riddings | Marston Lane End, Hilton | Results |
| 03 Jun 2007 | First Round | Marehay v Tutbury | The Maryfield, Ripley | Results |
| 03 Jun 2007 | First Round | Nutbrook v Ambergate | Nutbrook Sports Ground, West Hallam | Results |
| 03 Jun 2007 | First Round | Rolleston v Aston-on-Trent | Dovecliff Road, Rolleston-on-Dove | Results |
| 03 Jun 2007 | First Round | Shipley Hall v Chesterfield Second XI | The Field, Shipley | Results |
| 03 Jun 2007 | First Round | South Wingfield v Ilkeston Rutland | Holme Lane, South Wingfield | Results |
| 03 Jun 2007 | First Round | Stainsby Hall v Ockbrook and Borrowash Second XI | St Johns Road, Smalley | Results |
| 03 Jun 2007 | First Round | Ticknall v Spondon Second XI | The Grange, Ticknall | Results |
| 03 Jun 2007 | First Round | Wirksworth and Middleton v Pilsley | The Recreation Ground, Wirksworth | Results |
| 10 Jun 2007 | First Round | Sandiacre Town Second XI v Swarkestone | Longmoor Lane, Sandiacre | Results |
| 17 Jun 2007 | Second Round | Aston-on-Trent v Sandiacre Town Second XI | Shardlow Road, Aston-on-Trent | Results |
| 17 Jun 2007 | Second Round | Barton-under-Needwood v Ticknall | Holland Park Sports Club Ground, Barton-under-Needwood | Results |
| 17 Jun 2007 | Second Round | Belper Meadows v Marehay | Christchurch Meadows, Belper | Results |
| 17 Jun 2007 | Second Round | Matlock v Ockbrook and Borrowash Second XI | Causeway Lane, Matlock | Results |
| 17 Jun 2007 | Second Round | Rolls Royce v Ilkeston Rutland | Rolls Royce Ground, Derby | Results |
| 17 Jun 2007 | Second Round | Wirksworth and Middleton v Derby Congregational | The Recreation Ground, Wirksworth | Results |
| 04 Jul 2007 | Second Round | Denby Second XI v Hilton | The Copper Yard, Denby Village | Results |
| 06 Jul 2007 | Second Round | Chesterfield Second XI v Ambergate | Queen's Park, Chesterfield | Results |
| 08 Jul 2007 | Quarter-Final | Derby Congregational v Hilton | Brayfield Road, Littleover | Results |
| 08 Jul 2007 | Quarter-Final | Matlock v Ambergate | Causeway Lane, Matlock | Results |
| 08 Jul 2007 | Quarter-Final | Rolls Royce v Aston-on-Trent | Rolls Royce Ground, Derby | Results |
| 08 Jul 2007 | Quarter-Final | Ticknall v Belper Meadows | The Grange, Ticknall | Results |
| 29 Jul 2007 | Semi-Final | Aston-on-Trent v Ticknall | Shardlow Road, Aston-on-Trent | Results |
| 29 Jul 2007 | Semi-Final | Matlock v Derby Congregational | Causeway Lane, Matlock | Results |
| 27 Aug 2007 | Final | Matlock v Ticknall | Dovecliff Road, Rolleston-on-Dove | Results |
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