| 18 Apr 2010 | | Heyside v East Lancashire | Little Hey Street, Heyside | Results |
| 02 May 2010 | First Round | Baxenden v Longridge | Back Lane, Baxenden | Results |
| 03 May 2010 | First Round | Lytham v Monton and Weaste | Church Road, Lytham St Annes | Results |
| 09 May 2010 | First Round | Chorley v Walshaw | Windsor Park, Chorley | Results |
| 09 May 2010 | First Round | Stretford v New Brighton | The Boundary, Stretford | Results |
| 09 May 2010 | First Round | Ormskirk v St Annes | Brook Lane, Ormskirk | Results |
| 09 May 2010 | First Round | Farnworth Social Circle v Kashmir | Piggott Park, Farnworth | Results |
| 16 May 2010 | First Round | Greenmount v Edgworth | Brandlesholme Road, Greenmount | Results |
| 16 May 2010 | First Round | Wallasey v Heywood | The Kevin McCullagh Oval, Wallasey | Results |
| 16 May 2010 | First Round | Uppermill v Great Harwood | Leefields, Uppermill | Results |
| 16 May 2010 | First Round | Earby v Saddleworth | The Applegarth, Earby | Results |
| 16 May 2010 | First Round | Padiham v Winton | The Arbories Memorial Sports Ground, Padiham | Results |
| 16 May 2010 | First Round | Morecambe v Garstang | Woodhill Lane, Morecambe | Results |
| 16 May 2010 | First Round | Ramsbottom v Blackrod | Acre Bottom, Ramsbottom | Results |
| 16 May 2010 | First Round | Darcy Lever v Little Hulton | The Levers, Darcy Lever | Results |
| 16 May 2010 | First Round | Middleton v Swinton Moorside | Towncroft, Middleton | Results |
| 16 May 2010 | First Round | Denton West v Kearsley | Windsor Park, Denton | Results |
| 16 May 2010 | First Round | Egerton v Austerlands | Blackburn Road, Egerton | Results |
| 16 May 2010 | First Round | Glossop v Ramsey | North Road, Glossop | Results |
| 16 May 2010 | First Round | Netherfield v Great Eccleston | Parkside Road, Kendal | Results |
| 16 May 2010 | First Round | Read v Burscough | Whalley Road, Read | Results |
| 16 May 2010 | First Round | Preston v Penwortham | West Cliff, Preston | Results |
| 16 May 2010 | First Round | Woodhouses v Colwyn Bay | Medlock Road, Woodhouses | Results |
| 16 May 2010 | First Round | Horwich RMI v Vernon Carus | The Recreation Ground, Horwich | Results |
| 16 May 2010 | First Round | Castletown v Farnworth | King William's College, Castletown | Results |
| 16 May 2010 | First Round | Bury v Norden | Radcliffe Road, Bury | Results |
| 23 May 2010 | Second Round | Earby v Baxenden | The Applegarth, Earby | Results |
| 30 May 2010 | Second Round | Wallasey v Greenmount | The Kevin McCullagh Oval, Wallasey | Results |
| 31 May 2010 | Second Round | Vernon Carus v Bootle | Factory Lane, Penwortham | Results |
| 06 Jun 2010 | Second Round | Netherfield v Denton St Lawrence | Parkside Road, Kendal | Results |
| 06 Jun 2010 | Second Round | Preston v Woodhouses | West Cliff, Preston | Results |
| 06 Jun 2010 | Second Round | Read v St Annes | Whalley Road, Read | Results |
| 06 Jun 2010 | Second Round | Lytham v Norden | Church Road, Lytham St Annes | Results |
| 06 Jun 2010 | Second Round | Littleborough v Walshaw | Hare Hill, Littleborough | Results |
| 06 Jun 2010 | Second Round | Farnworth Social Circle v East Lancashire | Piggott Park, Farnworth | Results |
| 06 Jun 2010 | Second Round | Morecambe v Winton | Woodhill Lane, Morecambe | Results |
| 06 Jun 2010 | Second Round | Ramsbottom v Bamford Fieldhouse | Acre Bottom, Ramsbottom | Results |
| 06 Jun 2010 | Second Round | Middleton v Little Hulton | Towncroft, Middleton | Results |
| 06 Jun 2010 | Second Round | Denton West v Cheetham Hill | Windsor Park, Denton | Results |
| 06 Jun 2010 | Second Round | Glossop v Egerton | North Road, Glossop | Results |
| 06 Jun 2010 | Second Round | Hindley St Peters v Uppermill | St Peters Recreation Ground, Hindley | Results |
| 13 Jun 2010 | Second Round | New Brighton v Farnworth | Rake Lane, Wallasey | Results |
| 20 Jun 2010 | Third Round | Littleborough v Farnworth Social Circle | Hare Hill, Littleborough | Results |
| 20 Jun 2010 | Third Round | Greenmount v Uppermill | Brandlesholme Road, Greenmount | Results |
| 04 Jul 2010 | Third Round | Earby v Morecambe | The Applegarth, Earby | Results |
| 04 Jul 2010 | Third Round | Ramsbottom v Little Hulton | Acre Bottom, Ramsbottom | Results |
| 04 Jul 2010 | Third Round | Denton West v Glossop | Windsor Park, Denton | Results |
| 04 Jul 2010 | Third Round | Denton St Lawrence v Read | Sycamore Park, Denton | Results |
| 04 Jul 2010 | Third Round | Woodhouses v Vernon Carus | Medlock Road, Woodhouses | Results |
| 04 Jul 2010 | Third Round | Farnworth v Norden | Bridgeman Park, Farnworth | Results |
| 25 Jul 2010 | Quarter-Final | Uppermill v Littleborough | Leefields, Uppermill | Results |
| 25 Jul 2010 | Quarter-Final | Little Hulton v Earby | The Old Vicarage, Little Hulton | Results |
| 25 Jul 2010 | Quarter-Final | Denton St Lawrence v Denton West | Sycamore Park, Denton | Results |
| 29 Jul 2010 | Quarter-Final | Farnworth v Woodhouses | Bridgeman Park, Farnworth | Results |
| 15 Aug 2010 | Semi-Final | Little Hulton v Denton St Lawrence | The Old Vicarage, Little Hulton | Results |
| 15 Aug 2010 | Semi-Final | Farnworth v Littleborough | Bridgeman Park, Farnworth | Results |
| 12 Sep 2010 | Final | Denton St Lawrence v Farnworth | Vernon Road, St Annes | Results |
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